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Assay of glycogen phosphorylase in isolated fat cells of the rat

โœ Scribed by D.O. Allen; J. Ashmore


Book ID
115771404
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
566 KB
Volume
21
Category
Article
ISSN
0006-2952

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Radioactive method for the assay of glyc
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Glycogen phosphorylase (a-l,4-glucan:orthophosphate glueosyltransferase, EC 2.4.1.1) may be assayed in either the direction of glycogen synthesis or the direction of glycogen phosphorolysis. In the forward direction, phosphorolysis, progress of the reaction can be monitored continuously or as a fixe

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Hepatocytes were isolated from human liver tissue by a two-step perfusion technique. They were treated with vasopressin, angiotensin, ATP and phenylephrine, which are known to be Ca2 + -mediated glycogenolytic agents in rat liver tissue, and as a control, they were treated with the cyclic AMP-mediat