Assay of glycogen phosphorylase in isolated fat cells of the rat
โ Scribed by D.O. Allen; J. Ashmore
- Book ID
- 115771404
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 566 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0006-2952
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Glycogen phosphorylase (a-l,4-glucan:orthophosphate glueosyltransferase, EC 2.4.1.1) may be assayed in either the direction of glycogen synthesis or the direction of glycogen phosphorolysis. In the forward direction, phosphorolysis, progress of the reaction can be monitored continuously or as a fixe
Hepatocytes were isolated from human liver tissue by a two-step perfusion technique. They were treated with vasopressin, angiotensin, ATP and phenylephrine, which are known to be Ca2 + -mediated glycogenolytic agents in rat liver tissue, and as a control, they were treated with the cyclic AMP-mediat