Assay of digoxin in plasma by gas chromatography

✍ Scribed by Eric Watson; Sumner M. Kalman

• Book ID: 104145156
• Publisher: Elsevier Science
• Year: 1971
• Tongue: English
• Weight: 910 KB
• Volume: 56
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97802-6

✦ Synopsis

A method is described for the determination of digosin in llurnan plasma by gas chromatography with electron capture detection. The procedure involves addition of a labeled internal standard, ["Hldigosin, extraction with methylene cllloride and preliminary purification on a florisil column followed by thin-layer chromatography on silica gel. After formation of the heptafluorobutyrate derivative, the extract is rechromatographed on silica gel followed by gas-liquid chromatography.

The method has been applied to the determination of known amounts of digoxin ranging from S-100 ng per 10 ml plasma.