Assay of diamine oxidase by amperometric measurement of the rate of oxygen depletion
โ Scribed by Linda B. Marshall; Gary D. Christian
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 277 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0026-265X
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A dynamic method for free and total cholesterol assay based on the oxidation of cholesterol by cholesterol oxidase, and conversion of cholesteryl oleate to cholesterol by cholesterol esterase is discussed in this article. The reaction conditions for total cholesterol assay were a temperature of 310
The activity of diamine oxidase (DAO) in amniotic fluid was measured by three different methods. The first two, one calorimetric and the other fluorometric, depended on the coupled peroxidase assay. The other was a radiochemical procedure. The latter proved to be a reliable method, free from interfe