Assay of calmodulin with Bordetella pertussis adenylate cyclase

✍ Scribed by Alan Goldhammer; J. Wolff

• Book ID: 102984613
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 793 KB
• Volume: 124
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90217-2

✦ Synopsis

Low levels of the calcium-de~ndent regulator protein, calm~ulin, may be measured utilizing membranes prepared from 3ordetelIa pertussis which contain an adenylate cyclase which is activated by this protein. The activation is dose dependent and tissue levels of calmodulin can be determined over a range from 2 pg to 100 ng with good reliability. We demonstrate how this bioassay may be employed to measure the levels of calmodulin in a variety of protein and cellular preparations.