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Assay of ATP in intact cells of the facultative phototrophRhodobacter capsulatusexpressing recombinant firefly luciferase

โœ Scribed by Giovanna Di Tomaso; Roberto Borghese; Davide Zannoni

Book ID
105868244
Publisher
Springer
Year
2001
Tongue
English
Weight
122 KB
Volume
177
Category
Article
ISSN
0302-8933

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EDTA, pH 7.4 (Na,H,EDTA adjusted with NaOH or Na,EDTA adjusted with. HCl). EDTA is not completely soluble in this mixture, and the precipitated solid becomes finely dispersed in fresh mixtures. Large clumps form after 30 min of storage and E-E was therefore used fresh. On mixing E-E with sample (or