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Assay of adenylate cyclase by use of polyacrylamide-boronate gel columns

✍ Scribed by James H. Hageman; Glenn D. Kuehn

Book ID: 102628932
Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 481 KB
Volume: 80
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90678-9

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✦ Synopsis

A method is described for covalent attachment of up to 1.1 mmol of m-aminobenzeneboronic acid/g dry weight of polyacrylamide beads. Small (4 ml) columns of the derivatized beads have been used to separate quantitatively cyclic AMP from ATP in a single-step procedure. Columns of the polyacrylamideboronate gel have been used as the basis of a convenient new assay of the soluble adenylate cyclase prepared from nuclei of Physarum polycephalum.

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