Assay for Bacteria in Porous Media by Diffusion-Weighted NMR
✍ Scribed by K. Potter; R.L. Kleinberg; F.J. Brockman; E.W. Mcfarland
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 157 KB
- Volume
- 113
- Category
- Article
- ISSN
- 1064-1866
No coin nor oath required. For personal study only.
✦ Synopsis
In this work, an NMR technique capable of detecting bacterial bution can be performed in the field, using NMR well-logcells and measuring the cell density in suspension and in porous ging equipment (2, 3).
media has been developed. It is based on the pulsed-field-gradient
Bacteria used in the bioremediation process are typically technique and relies on the fact that extracellular water diffuses about 1 mm in diameter and the density of cells ranges from freely while intracellular water is completely restricted by the rela-10 6 to 10 9 cells per gram of sediment or soil. Therefore, the tively impermeable cell wall of the bacterium. At high wave vec-NMR signal from the intracellular water protons is likely to tors, the signal from extracellular water is completely suppressed be overwhelmed by the signal from surrounding water. Since while the signal from intracellular water is comparatively unafextracellular water diffuses comparatively freely in contrast fected. This technique has been applied to the mapping of bacterial to intracellular water, it is possible to use a diffusion filter distributions in porous media. This method is presented as a nonto eliminate the signal from extracellular water. This is roudestructive, real-time technique for biomass characterization within laboratory column and flow cell experiments, and possibly tinely used to acquire spectroscopic information of intracelfor monitoring in situ bioremediation. ᭧ 1996 Academic Press, Inc.
lular components (4). Recently, diffusion-weighted imaging was also used to map the distribution of mammalian cells in a hollow fiber bioreactor; however, NMR measurements