๐”– Bobbio Scriptorium
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Aspects of inorganic nitrogen assimilation in yeasts

โœ Scribed by Valerie J. Burn; Penelope R. Turner; C. M. Brown

Publisher
Springer Netherlands
Year
1974
Tongue
English
Weight
543 KB
Volume
40
Category
Article
ISSN
0003-6072

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โœฆ Synopsis

Cultures of Candida utilis utilise glutamate in preference to ammonia and ammonia in preference to nitrate. The nitrate reductase of this organism is induced by nitrate and repressed in cultures grown on glutamate or ammonia. Nitrate-grown cultures of C. utilis, irrespective of the medium nitrate concentration, behave as though nitrogen-limited. In contrast to C. utilis, Saccharomyces cerevisiae utilises ammonia in preference to glutamate.

In eight yeasts studied the highest cellular contents of biosynthetic NADPlinked glutamate dehydrogenase were found in batch cultures containing low concentrations of ammonia or in nitrogen-limited chemostat cultures. NADlinked glutamate dehydrogenase activity was detected in extracts of cells grown in the presence of glutamate but not in those grown in the presence of ammonia.

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