Aspects of aerobic mineralization during spring in Lake Vechten with special reference to the14C-labelling technique
✍ Scribed by J. J. Olie; Th. E. Cappenberg
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 634 KB
- Volume
- 95
- Category
- Article
- ISSN
- 1573-5141
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✦ Synopsis
Aerobic mineralization, i .e . seston respiration, microbial breakdown of detritus and microbial assimilationdissimilation of photosynthetically derived D(issolved) O(rganic) C(arbon) was measured in concentrated samples from the pelagic zone of Lake Vechten . The samples were described by cell numbers of dominant algae prior to concentrating by centrifuge with continuous rotor . The concentrated samples were incubated in the laboratory at in situ temperature and a light intensity of 30 W . m-2 for measuring primary production and photosynthetically derived DOC . After filtration the particulate fraction was incubated in unlabelled lake water for measuring respiratory production of DI 14 C . Portions of the same particulate fraction were sterilized and incubated in unlabelled sample concentrate for measuring microbial breakdown of detritus . The seston respiration amounted to 19-30% loss of the particulate fraction over 15-45 h . Microbial breakdown of detritus amounted to 28-40% loss of the particulate fraction over 24-168 h . In both cases P(articulate) O(rganic) 14 C(arbon) was transformed to mainly D1 14 C . Microbial assimilation-dissimilatioh of photosynthetically derived DOC could not be measured reliably .