Ascorbic acid effects on serotonin fluorescence

✍ Scribed by J.H. Thompson; C.A. Spezia; M. Angulo

• Book ID: 102627672
• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 495 KB
• Volume: 31
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(69)90272-3

✦ Synopsis

Serotonin is highly unstable. It may be totally destroyed during assay by dissolved oxygen, by iron contaminants or monoamine oxidases during extraction from biological specimens, or by iron and UV irradiation during its fluorometric detection. Various investigators have used EDTA, ascorbic acid, nitrogen, and monoamine oxidase inhibitors to control loss during extraction (l-5). Ascorbic acid may be used successfully during detection but we have found that stability is accompanied by diminished serotonin fluorescence (6).