Artificial insertion of a dominant gene for resistance to avian leukosis virus into the germ line of the chicken
✍ Scribed by D. W. Salter; L. B. Crittenden
- Book ID
- 104697588
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 465 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0040-5752
No coin nor oath required. For personal study only.
✦ Synopsis
This report describes the unique biological properties of a transgenic chicken line that contains a defective avian leukosis virus (ALV) proviral insert that we call alv6. Chick embryo fibroblasts (CEF) containing this insert express subgroup A envelope glycoprotein since they yield focus-forming pseudotype virus when co-cultivated with transformed quail cells expressing envelope-defective Bryan high-liter Rous sarcoma virus (RSV). In addition, these cells display high interference to subgroup A RSV but not to subgroup B RSV infection. Chickens containing this insert are highly resistant to pathogenic subgroup A ALV infection, but show little immunological tolerance to subgroup B ALV infection. Thus we have artificially inserted a dominant gene for resistance to avian leukosis infection into the chicken germ line.