Arterial expression of 5-HT2Band 5-HT1Breceptors during development of DOCA-salt hypertension

✍ Scribed by Amy KL Banes; Stephanie W Watts

• Publisher: BioMed Central
• Year: 2003
• Tongue: English
• Weight: 764 KB
• Volume: 3
• Category: Article
• ISSN: 1471-2210
• DOI: 10.1186/1471-2210-3-12

✦ Synopsis

Background: 5-hydroxytryptamine (5-HT) 2B and 5-HT 1B receptors are upregulated in arteries from hypertensive DOCA-salt rats and directly by mineralocorticoids. We hypothesized that increased 5-HT 2B and 5-HT 1B receptor density and contractile function would precede increased blood pressure in DOCA-high salt rats. We performed DOCA-salt time course (days 1, 3, 5 and 7) studies using treatment groups of: DOCA-high salt, DOCA-low salt, Sham and Sham-high salt rats.

Results:

In isolated-tissue baths, DOCA-high salt aorta contracted to the 5-HT 2B receptor agonist BW723C86 on day 1; Sham aorta did not contract. The 5-HT 1B receptor agonist CP93129 had no effect in arteries from any group. On days 3, 5 and 7 CP93129 and BW723C86 contracted DOCAhigh salt and Sham-high salt aorta; Sham and DOCA-low salt aorta did not respond. Western analysis of DOCA-high salt aortic homogenates revealed increased 5-HT 2B receptor levels by day 3; 5-HT 1B receptor density was unchanged. Aortic homogenates from the other groups showed unchanged 5-HT 2B and 5-HT 1B receptor levels.

Conclusion:

These data suggest that functional changes of 5-HT 2B but not 5-HT 1B receptors may play a role in the development of DOCA-salt hypertension.