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Arrest of 3T3 cells in G1 phase in suspension culture

โœ Scribed by Haruki Otsuka; Merwin Moskowitz

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
453 KB
Volume
87
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

Abstract

3T3 cells do not grow in Methocel suspension culture, while other permanent cell lines do. The viability of 3T3 cells in suspension remains unchanged for at least three days with respect to plating efficiency, vital staining and resumption of normal growth when transferred into monolayer culture. When monolayer 3T3 cells in G1 phase are suspended they remain in G1 phase. Cells already in S phase which are suspended complete ongoing DNA synthesis and mitosis and then are arrested in the G1 phase. Progress through the cell cycle is reinitiated after suspended cells attach to a surface. When monolayer cells in late G1 phase (just before entering S phase) are put in suspension cultures they do not initiate DNA synthesis.

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