Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene
✍ Scribed by Shelly R. Gunn; Aswani R. Bolla; Lynn L. Barron; Mercedes E. Gorre; Mansoor S. Mohammed; David W. Bahler; Clemens H.M. Mellink; Marinus H.J. van Oers; Michael J. Keating; Alessandra Ferrajoli; Kevin R. Coombes; Lynne V. Abruzzo; Ryan S. Robetorye
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 388 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0145-2126
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✦ Synopsis
We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to approximately 1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.