Arginine-rich polypeptides in saline extract of chlorella pyrenoidosa- 14C
✍ Scribed by Zdenek Nejedly; Karel Hybs
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- French
- Weight
- 375 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
The radiochromatographic analysis of the hot 10% sodium chloride extract of the alga Chlorella pyrenoidosa‐ ^14^C indicated the presence of compounds rich in L‐arginine, besides of nucleic acids and glucose‐type polysaccharide estimated previously. L‐Arginine is present in a bound form, most likely as a protamine‐type polypeptide or a histone‐type protein containing L‐arginine as a dominant component, besides of smaller amounts of L‐glutamic acid, L‐aspartic acid and L‐serine, A simple procedure of the isolation of L‐arginine‐ ^14^C/U/ from the saline extract of radioactive Chlorella cells, on a preparative scale, is described.