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Are we analysing knockdown in the right way? how independence of the knockdown-recovery process from mortality may affect measures for behavioural effects in pesticide bioassays

✍ Scribed by Jagers op Akkerhuis, Gerard A J M; Seidelin, N; Kjaer, Christian


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
273 KB
Volume
55
Category
Article
ISSN
1526-498X

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✦ Synopsis


In pesticide bioassays, especially those with neurotoxic agents, eþ ects on animals are typically grouped into classes according to behaviour, such as normal and aþ ected behaviour, which may range from unstable walking behaviour, to unable to move, to mortality. Generally, recovery is observed in all these eþ ect classes, except the last. Mortality, however, disturbs the analysis of the recovery processes because it decreases the number of animals that otherwise could have shown a reversible eþ ect. We consider that this interaction between mortality and other, reversible, eþ ects is a conceptual problem, and give arguments in favour of analysing changes in behaviour and mortality as two independent, simultaneously occurring neurotoxic syndromes. As an illustration, two data sets are analysed in both ways and these show that marked diþ erences may exist between conclusions reached by the two viewpoints. The consequences thereof are discussed in relation to toxico-kinetic explanations for neurotoxicant eþ ects on behaviour and mortality.