Arachidonic acid metabolism by adult human osteoblast-like cells exhibits sexually dimorphic characteristics
✍ Scribed by Philip E. Keeting; Chun Hong Li; Madhavi Murty; Jiaquan Xu; David S. Cissel; Diana L. Whipkey; Geoffrey M. Graeber; J. David Blaha
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 90 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
The eicosanoids, including prostaglandin E 2 (PGE 2 ) and other bioactive arachidonic acid metabolites, are important local mediators of bone remodeling. Presumably, the limited or excessive synthesis of the eicosanoids could compromise bone homeostasis. We have noted that the stimulated release of arachidonic acid by adult male donor derived human osteoblast-like (hOB) cells exceeded the stimulated release measured for female-derived hOB cells by 1.5-fold. Assays of PGE 2 biosynthesis by cytokine-stimulated hOB cells also demonstrated a sex-linked difference, such that male hOB cell PGE 2 production exceeded female cell production by 1.6-2.2-fold. The calciumdependent cytoplasmic phospholipase A 2 activity in subcellular fractions prepared from hOB cell homogenates was higher in both the cytosolic (1.6-fold) and particulate (1.5-fold) fractions from the male cells than in those prepared from female hOB cells, suggesting a molecular basis for the observed sexually dimorphic characteristics related to arachidonic acid metabolism by hOB cells. The relatively limited capacity of the female cells may limit needed intracellular and intercellular signaling during bone remodeling, thereby contributing to the development of bone pathology.