Arachidonic acid metabolic profiles in human meningiomas and gliomas

✍ Scribed by Maria Grazia Castellil; Giorgio Butti; Chiara Chiabrando; Elena Cozzi; Roberto Fanelli; Paolo Gaetani; Vittorio Silvani; Pietro Paoletti

• Book ID: 104642287
• Publisher: Springer US
• Year: 1987
• Tongue: English
• Weight: 430 KB
• Volume: 5
• Category: Article
• ISSN: 0167-594X
• DOI: 10.1007/bf00148394

✦ Synopsis

We determined arachidonic acid (AA) cyclooxygenase metabolic profiles in specimens of human intracranial tumors (gliomas and meningiomas) and, when available, normal brain tissue. Samples were collected at surgery and immediately frozen in liquid nitrogen. The five stable metabolites of AA (PGE2, PGD2, PGF2~, 6-keto-PGFlc~ and TXB2) were measured by high-resolution gas chromatography-mass spectrometry after ex vivo metabolism of endogenous AA by tissue homogenates. The absolute amounts of AA metabolites varied widely between samples, though meningiomas and gliomas showed characteristic profiles. Compared to the slowgrowing benign meningiomas, the rapidly-growing infiltrating gliomas had higher synthesis of TXA2 (reported as a procancer metabolite) and lower synthesis of PGD2 and PGI2 (reported as anticancer metabolites). A higher overall synthesis capacity, preferentially toward TXA2, was found in glioblastomas than in nonpathological brain tissue.