Aqueous two-phase systems with a liquid protein (bovine serum albumin) phase for partitioning of enzymes
✍ Scribed by Göte Johansson
- Publisher
- Elsevier Science
- Year
- 1996
- Weight
- 436 KB
- Volume
- 680
- Category
- Article
- ISSN
- 0378-4347
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📜 SIMILAR VOLUMES
During recombinant E. coli fermentation with high-expression levels inclusion bodies are often formed. Aqueous two-phase systems have been successfully used in the presence of urea for the initial recovery step of inclusion bodies from E. coli. Basic studies of the complex interactions are lacking.
Genetic engineering has been used for the fusion of peptides, with different length and composition, on a protein to study the effect on partitioning in aqueous two-phase systems containing thermoseparating polymers. Peptides containing 2-6 tryptophan residues or tryptophan plus 1-3 lysine or aspart