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Aqueous Two-Phase Systems Containing Urea: Influence on Phase Separation and Stabilization of Protein Conformation by Phase Components

✍ Scribed by Christian Rämsch; Lutz B. Kleinelanghorst; Esther A. Knieps; Jörg Thömmes; Maria-Regina Kula

Book ID
111522782
Publisher
American Institute of Chemical Engineers
Year
1999
Tongue
English
Weight
95 KB
Volume
15
Category
Article
ISSN
8756-7938

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Aqueous two-phase systems containing ure
Aqueous two-phase systems containing urea: Influence of protein structure on protein partitioning
✍ Christian Rämsch; Lutz B. Kleinelanghorst; Esther A. Knieps; Jörg Thömmes; Maria 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 200 KB 👁 2 views

During recombinant E. coli fermentation with high-expression levels inclusion bodies are often formed. Aqueous two-phase systems have been successfully used in the presence of urea for the initial recovery step of inclusion bodies from E. coli. Basic studies of the complex interactions are lacking.