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Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

✍ Scribed by Richard B. Sheridan III; P.C. Huang

Book ID
113191337
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
417 KB
Volume
52
Category
Article
ISSN
0027-5107

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## Abstract We used a shuttle vector based on the Epstein‐Barr virus origin of plasmid replication (oriP) to determine the types of mutations induced by depurination in human cells. Plasmid DNA was incubated at pH 2 at 40Β°C for various times to induce up to 20 apurinic (AP) sites per 9.7‐kb plasmid