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Aptamer–oligonucleotide binding studied by capillary electrophoresis: Cation effect and separation efficiency

✍ Scribed by Claire André; Alain Xicluna; Yves-Claude Guillaume

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 218 KB
Volume: 26
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200500170

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## Abstract Binding stoichiometries between four DNA aptamers (RT12, RT26, RTlt49, and ODN93) and the reverse transcriptase (RT) of the type 1 human immunodeficiency virus (HIV‐1) were studied using affinity CE (ACE) coupled with LIF polarization and fluorescence polarization (FP). The ACE/LIF stud