Approaches to site-directed DNA integration based on transposases and retroviral integrases

Site-Directed Spin-Labeling of DNA by th

Site-Directed Spin-Labeling of DNA by the Azide–Alkyne ‘Click’ Reaction: Nanometer Distance Measurements on 7-Deaza-2′-deoxyadenosine and 2′-Deoxyuridine Nitroxide Conjugates Spatially Separated or Linked to a ‘dA-dT’ Base Pair

✍ Ping Ding; Dorith Wunnicke; Prof. Heinz-Jürgen Steinhoff; Prof. Frank Seela 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 674 KB

## Abstract Nucleobase‐directed spin‐labeling by the azide‐alkyne ‘click’ (CuAAC) reaction has been performed for the first time with oligonucleotides. 7‐Deaza‐7‐ethynyl‐2′‐deoxyadenosine (**1**) and 5‐ethynyl‐2′‐deoxyuridine (**2**) were chosen to incorporate terminal triple bonds into DNA. Oligon