Applied Fluxomics for Metabolic Engineering of Corynebacterium glutamicum
✍ Scribed by A. Marx; S. Hans; B. Bathe; S. Petersen; A. A. de Graaf; B. Möckel
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- German
- Weight
- 33 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0009-286X
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The physiology and central carbon metabolism of Corynebacterium glutamicum was investigated through the study of specific disruption mutants. Mutants deficient in phosphoenolpyruvate carboxylase (PPC) and/or pyruvate kinase (PK) activity were constructed by disrupting the corresponding gene(s) via t
To evaluate the importance of reactions within the central metabolism under different flux burdens the fluxes within the pentose phosphate pathway (PPP), as well as the other reactions of the central metabolism, were intensively analyzed and quantitated. For this purpose, Corynebacterium glutamicum