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Applications of LC/MS in structure identifications of small molecules and proteins in drug discovery

✍ Scribed by Guodong Chen; Birendra N. Pramanik; Yan-Hui Liu; Urooj A. Mirza

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 263 KB
Volume: 42
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.1184

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

With advancements in ionization methods and instrumentation, liquid chromatography/mass spectrometry (LC/MS) has become a powerful technology for the characterization of small molecules and proteins. This article will illustrate the role of LC/MS analysis in drug discovery process. Examples will be given on high‐throughput analysis, structural analysis of trace level impurities in drug substances, identification of metabolites, and characterization of therapeutic protein products for process improvement. Some unique MS techniques will also be discussed to demonstrate their effectiveness in facilitating structural identifications. Copyright © 2007 John Wiley & Sons, Ltd.

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