## Abstract Isothermal titration calorimetry (ITC) is a fast, accurate and label‐free method for measuring the thermodynamics and binding affinities of molecular associations in solution. Because the method will measure any reaction that results in a heat change, it is applicable to many different
Applications of Isothermal Titration Calorimetry in the Characterization of the Energetics, and Kinetics, of the Interaction of Amphiphiles with Lipid Bilayers
✍ Scribed by Moreno, Maria Joao; Martins, Patrícia T.; Velazquez-Campoy, Adrian
- Book ID
- 122109849
- Publisher
- Biophysical Society
- Year
- 2013
- Tongue
- English
- Weight
- 39 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0006-3495
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Isothermal titration calorimetry (ITC) is now an established and invaluable method for determining the thermodynamic constants, association constant and stoichiometry of molecular interactions in aqueous solutions. The technique has become widely used by biochemists to study protein interaction with
The principles of isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) are reviewed together with the basic thermodynamic formalism on which the two techniques are based. Although ITC is particularly suitable to follow the energetics of an association reaction between b