Applications of chromic acid-celite columns to lipid analysis. Location of double bond position in submicro- and microgram amounts of methyl octadecenoates
✍ Scribed by Daniel P. Schwartz
- Book ID
- 102983827
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 549 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A procedure for locating the double bond position in a series of methyl octadecenoates is detailed. Submicrogram or microgram amounts of substrate dissolved in CSZ are brought in contact with a very small column of chromic acid on Celite, and the oxidation products (carboxylic acids) are eluted, converted to methyl esters, and resolved by gas-liquid chromatography. Beside the acids resulting from scission of the double bond, acids containing one less carbon atom arise from oxidation of the allylic carbons on both sides of the double bond so that pairs of peaks appear on the chromatogram. All positions from A3 to Al7 were located successfully. The A2 position failed to oxidize.