Applications of affinity interactions in capillary electrophoresis
β Scribed by Niels H. H. Heegaard
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 120 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0173-0835
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π SIMILAR VOLUMES
Molecular recognition may be characterized both qualitatively and quantitatively by electrophoretic methods if complexed molecules differ in electrophoretic mobility from unbound ones. The use of capillary zone electrophoresis (CE) for the characterization of affinity interactions is advantageous be
Capillary affinity electrophoresis was used to study the interactions of lectins from Tetragonolobus purpureas with L-(-)-fucose 1-phosphate. The electrophoretic mobilities of three lectin peaks were influenced by the complexation with the negatively charged fucose derivative. A model that allows th