Application ofin vivo bioluminescence to the study of lonophoretic action

✍ Scribed by Simpson, W. J.

• Book ID: 101717097
• Publisher: John Wiley and Sons
• Year: 1993
• Weight: 370 KB
• Volume: 8
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170080304

✦ Synopsis

lonophores (carbonyl cyanide m-chlorophenylhydrazone; valinomycin; and the hopderived compounds colupulone, trans-isohumulone and trans-humulinic acid) reduced the rate of dodecanal-dependent light emission from /uxA/B-transformed cells of Lactococcus lactis subsp. diacetylactis F712 when the cells were suspended in a buffered medium (pH 6.4) containing glucose. This allowed an assay for ionophores t o be devised and permitted measurement of the effects of such compounds on the test organism by the use of a non-destructive technique in real time.