Application of UV absorbance and second-derivative spectrophotometry for analysing casein hydrolysates

✍ Scribed by Marialice P.C. Silvestre; Chantal Dauphin; Michel Hamon

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 823 KB
• Volume: 282
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80125-5

✦ Synopsis

W absorbance and second-derivative spectrophotometry were used to analyse some tryptic and pancreatic casein hydrolysates. The decrease in absorptivities detected at the initial stage of protein hydrolysis indicated some similarities with the denaturation process, probably owing to the rupture of hydrophobic bonds. This technique was used to determine the degree of hydrolysis and the homogeneity of such preparations and was shown to be useful as a complementary method in characterizing casein hydrolysates. Tryptophan addition, used to compensate for the losses during the fabrication of protein hydrolysates, could also be detected by this technique.