Application of urease conductometric biosensor for heavy-metal ion determination

✍ Scribed by G.A. Zhylyak; S.V. Dzyadevich; Y.I. Korpan; A.P. Soldatkin; A.V. El'skaya

• Book ID: 103961215
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 358 KB
• Volume: 24
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/0925-4005(95)85031-7

✦ Synopsis

Urease conductometric

biosensors consisting of interdigitated gold electrodes and enzyme membranes formed on their sensitive parts have been used for a quantitative estimation of general water pollution with heavy-metal ions. The measurements of the urease residual activity have been carried out in Tris-HNO, buffer after preincubation in model metal-salt solution.

The detection limits, depending on preincubation time and dynamic ranges, have been determined in model solutions of heavymetal ions. The sequence of metals ions relative to their toxicity toward urease is: Hg'+ > Cu*' > Cd*+ > Co'+ > Pb2+ > S?+, The conditions for practical applications of the biosensors have been investigated and critically evaluated for optimization. Urease reactivation by EDTA after inhibition by heavy-metal ions has been demonstrated. The performance characteristics of the conductometric biosensor are discussed.