Application of turbulent flow chromatography to the metabonomic analysis of human plasma: Comparison with protein precipitation

✍ Scribed by Filippos Michopoulos; Antony M. Edge; Georgios Theodoridis; Ian D. Wilson

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 287 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900789

✦ Synopsis

Abstract

The use of turbulent flow chromatography (TFC) as a method for the rapid metabonomic LC‐MS analysis of plasma as an alternative to solvent‐based protein precipitation has been investigated. This comparison has shown that TFC can be effectively used in this application with the benefit that off‐line sample handling is significantly reduced. However, analysis of the data obtained via TFC for human plasma reveals substantial differences in the overall metabolite profiles compared with methanol‐precipitated HPLC‐MS. This seems in part at least to be related to greatly reduced amounts of phospholipids (ca. 10 fold reduction) for the turbulent flow methodology compared with protein‐precipitated samples. The significance of these differences with respect to metabolite profiles as a result of the sample preparation method used are discussed.