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Application of the Davidon-Fletcher-Powell algorithm to the resolution of multicomponent mixtures using UV-vis spectrophotometry

✍ Scribed by M. Blanco; J. Coello; J. Gené; H. Iturriaga; S. Maspoch; A.R. Puigdomènech


Book ID
102985969
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
595 KB
Volume
327
Category
Article
ISSN
0003-2670

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✦ Synopsis


The Davidon-Fletcher-Powell (DPP) iterative algorithm was used for the simultaneous quantitation of the components of complex mixtures from their UV-vis spectra. First, the effect of noise, overlap between standard spectra and the starting point for resolving numerically generated spectra were investigated. Then, spectra for the pure solutions of the mixture components were used to apply the method to the resolution of ternary and quatemary mixtures of active principles and pharmaceutical preparations. Samples were also quantified by using a set of standard spectra which included not only those of the pure components, but also some for other substances. The proposed method effectively quantifies the actual mixture components.