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Application of the Commercial Gel Electrophoresis Apparatus with Intermittent Fluorescence Scanning to a Nonfluorescing Protein

✍ Scribed by Nong Chen; Andreas Chrambach

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
183 KB
Volume
242
Category
Article
ISSN
0003-2697

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A new method for visualizing 2D protein spot patterns is described whereby proteins containing sulfhydryl groups are labeled with the fluorophore monobromobimane prior to the isoelectric focusing step of 2D polyacrylamide gel electrophoresis. The method requires the addition of a single reagent and