Application of the cell block method, utilizing mount quick mounting medium
✍ Scribed by Chiyuki Kaneko; Masanori Funahashi; Kazuo Kato; Tadao K. Kobayashi
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 269 KB
- Volume
- 22
- Category
- Article
- ISSN
- 8755-1039
No coin nor oath required. For personal study only.
✦ Synopsis
Our objective was to determine the applicability of cell transfer and cell block methods using Mount Quick (Daido Sangyo, Saitama, Japan) mounting medium (MQ) for hematoxylin-eosin (H&E) and immunohistochemical staining of several limited amounts of biological materials in slide preparations.
The materials investigated were histopathologically confirmed malignant mesotheliomas (pleural effusions) and malignant lymphomas, a malignant melanoma, and an amelanotic melanoma in sealed slides. Monoclonal antibodies against carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), cancer antigen 125 (CA-125), vimentin, thrombomodulin (TM), cytokeratin, UCHL-1, L-26, melanoma-specific antigen (HMB45), and S-100 protein (S-100) were applied in the investigation.
The malignant mesotheliomas were found to be positive for EMA, cytokeratin, vimentin, TM, and CA-125, and negative for CEA, with no differences being observed in findings from direct contact preparations. Using T-cell-type malignant lymphomas for immunohistochemistry, UCHL-1 positivity and L-26 negativity were clearly demonstrated.
The malignant melanoma and amelanotic melanoma materials stained strongly for HMB45 and S-100.
Cell transfer employing MQ is a suitable approach for immunohistochemical investigations of limited materials. In addition, cell blocks derived from MQ-embedded smears can be used for both H&E and immunohistochemical staining. Diagn.