Application of statistical selection procedures for selecting the mutant with the highest enzyme activity from a set of mutants of the species Aspergillus niger

Selection of the most active enzymes fro

Selection of the most active enzymes from a mixture of phage-displayed β-lactamase mutants

✍ Sophie Vanwetswinkel; Jacqueline Marchand-Brynaert; Jacques Fastrez 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 238 KB

A mixture of phages displaying the wild type and four mutant Rtem 13-1actamases was submitted to three rounds of selection by incubation with a biotinylated suicide inhibitor followed by chromatography on streptavidin coated beads. The final mixture of selected phages was shown to be made up of two