β Scribed by Yuka Miyazawa; Naoko Sakai; Naoyuki Murakami; Tetsuya Konishi
No coin nor oath required. For personal study only.
A liquid scintillation counting method for simultaneous determination of three radioactive nuclides (3H, 14C, and 22Na) of biological interest was studied. By comparing the beta spectra of the three nuclides, their counting energy ranges, A, B, and C, were determined. 22NA was set high enough to avoid any spillover counts from lower-energy nuclides. Region A for 3H was set to maximize the counting efficiency. A good correlation between the counting efficiency for 22Na in region C and the counting efficiency of other nuclides in all regions was obtained. Prior to 3H and 14C dpm calculations, the 22Na counts spilled down in regions A and B were subtracted from the total counts in regions A and B. A simple linear equation was then used to compute 3H and 14C dpm. Findings show that the method presented is adaptable for highly quenched samples up to quenching indices of tSIE = 100. The method is useful for studying the biological transport coupled to Na+.
π SIMILAR VOLUMES
The organic samples triply labeled with 3H, 14C, and 35S were combusted in a flow-type combustion flask under a continuous oxygen flow. The oxygen gas containing the combustion products, which were mainly 3H2O, 14CO2, and 35SO2, was introduced into successive 0.5 M H2O2 and Oxisorb (2-methoxyethylam