Application of Sepharose and Sephadex modified by means of radiation grafting in separation of biomolecules

✍ Scribed by Li Jun; Yi Min; Ha Hongfei

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 191 KB
• Volume: 46
• Category: Article
• ISSN: 0969-806X
• DOI: 10.1016/0969-806x(95)00275-3

✦ Synopsis

In this work Sepharose-NASI-BSA and Sephadex-NASI-BSA were prepared by means of preirradiation grafting and direct radiation grafting separately. The radiation grafting condition were given.

In order to get those compounds the conjugation reaction between NAS] and BSA was investigated as well. The reaction was performed at 37 "C for 2ha, but in the case of Sephadex-NASI,it took longer time,6hs,to finish the conjugation reaction.

The application of the radiation modified matrixes as a kind of filling materials of column chromatography in solmration of D,L-tryptoph~ was studied. The results showed that this method was accessible after some improvements.