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Application of Semi-Selective Excitation Sculpting for Homonuclear Decoupling During Evolution in Multi-Dimensional NMR

✍ Scribed by V. V. Krishnamurthy

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
235 KB
Volume
35
Category
Article
ISSN
0749-1581

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✦ Synopsis

A band-selective homonuclear decoupled TOCSY sequence is described and compared with the standard TOCSY experiment, using a sample of a DNA 15mer. Band selection and homonuclear decoupling (BASHD) was achieved along the evolution dimension using a band-selective double pulse Ðeld gradient spin echo (DPFGSE) technique. This semi-selective excitation sculpting technique substantially improves the resolution and sensitivity of a crowded region in the TOCSY spectrum of the DNA 15mer chosen. The methodology described is general and can be implemented in other 2D and 3D techniques to achieve band selection and homonuclear decoupling during proton evolution.

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