A method that allows capillary electrophoresis to be used as a microconcentrating technique is presented. An 85-fold improvement in the amount of material that can be injected into a capillary column without loss of resolution is shown. The method can be used for negative- and positive-charged speci
Application of sample stacking to gravity injection in capillary electrophoresis
✍ Scribed by Dean S. Burgi; Ring-Ling Chien
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 341 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
Abstract
Sample stacking can be used to increase the amount of analyte loaded onto a capillary column without loss in resolution. After the sample is loaded onto the column, the analytes are then separated by conventional capillary zone electrophoresis. When using gravity injection, over an order of magnitude improvement in detection limit is achieved. There is an optimal point for the amount of material which can be injected onto the column based on the resolution desired for the separation.
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