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Application of (S)-TBMB carboxylic acid-based on-line HPLC-exciton CD analysis to acyclic vicinal alcohols and amino alcohols

✍ Scribed by Kumi Shimizu; Jeong-Hwan Kim; Kazuaki Akasaka; Hiroshi Ohrui


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
448 KB
Volume
11
Category
Article
ISSN
0899-0042

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✦ Synopsis


Applications of the on-line HPLC-exciton CD analysis using (S)-2-tertbutyl-2-methyl-1,3-benzodioxole-4-carboxylic acid [(S)-TBMBC-OH] that can simultaneously determine the enantiomeric compositions and the absolute configuration of cyclohexane-1,2-diols and diamines as well as acyclic vicinal diols and amino alcohols were studied. Di-O-or di-N,O-(S)-TBMBC derivatives of acyclic terminal vicinal diols, 2-hydroxy-1-amines, and nonterminal vicinal diols gave symmetrical exciton CD spectra between enantiomers, indicating their absolute configurations. However, Di-N,O-(S)-TBMBC derivatives of 2-amino-1-ols did not always give symmetrical exciton CD spectra between enantiomers, but their 2-phthalimido-1-O-(S)-TBMBC derivatives gave symmetrical exciton CD spectra, indicating their absolute configurations. All these (S)-TBMBC derivatives were separated by normal-phase HPLC and unequivocally determined by the on-line HPLC-exciton CD analysis without recourse to reference samples.