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Application of reversed phase liquid chromatography in the analysis of saponins in faba bean

✍ Scribed by Amarowicz, R. ;Shimoyamada, M. ;Okubo, K.

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 151 KB
Volume: 35
Category: Article
ISSN: 0027-769X
DOI: 10.1002/food.19910350220

No coin nor oath required. For personal study only.

✦ Synopsis

Due to the high biological activity of saponins this group of compounds has become the object of interest of many researchers from different scientific disciplines [7]. In the analysis of saponins the stage of isolation of an investigated compound which follows the extraction from the plant material is decisive. Liquid column chromatography, e. g. ion-exchange chromatography [ 121, silica gelchromatography [I, 4,5, 111, gel filtration on Sephadex LH-20 [6,9, 101 and reversed phase chromatography have been used in the procedure. In the case of the latter columns were filled with octasilane [S] or octadecylsilane (ODS) bonded to silica gel [2, 4, 111.

The aim of the study was to apply reversed phase liquid chromatography in the analysis of saponins in faba bean.

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