Application of Polymer-Protected Ultrafine Platinum Particles to the Immunological Detection of Human Serum Albumin
✍ Scribed by T. Uda; R. Kanmei; S. Akasofu; E. Hifumi; M. Ohtaki
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 607 KB
- Volume
- 218
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The immunological properties of ultrafine platinum particles protected with poly(methyl acrylate)-co-N-vinyl-2-pyrrolidone, having a group chemically reactive with protein, were examined. Polymer-protected ultrafine particles were able to chemically bond multiple human serum albumin (HSA) molecules on their surface. The particles behaved as a multi-epitope-type antigen for the immunoreaction. In an ELISA, the HSA-bound particles and HSA molecules competitively reacted against the anti-HSA monoclonal antibodies. The limit of detection of HSA was ca. 10 ng/ml in the assay. The particles were used in an immunodot analysis of anti-HSA polyclonal antibody. Within 30 min of the reaction, 1-10 micrograms/ml of HSA was detected. The protein fixed polymer-protected ultrafine particles are a useful new material in immunochemistry.