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Application of polyethyleneimine cellulose for the class separation of steroidal carboxylic acids from neutral steroids and pigments in urine

✍ Scribed by Carl Monder; Francisca Iohan


Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
498 KB
Volume
139
Category
Article
ISSN
0003-2697

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✦ Synopsis


Metabolites of corticosteroids that contain the 2 1 -oic acid moiety are found in human urine. The acids from neutral steroids and urinary pigments have been separated by passing the mixture through a column of polyethyleneimine cellulose. The acids adhering to the column are quantitatively eluted with dilute formic acid. The purified preparation is suitable for derivatization and chromatographic analysis.