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Application of photochemical reactions of bis-azido compounds to preparation of an enzyme-polymer film

✍ Scribed by Sachio Miyairi; Hideaki Tanaka; Masaaki Sugiura; Sakuzo Fukui

Book ID: 102627091
Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 215 KB
Volume: 97
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90079-4

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✦ Synopsis

3-Glucosidase (EC 3.2.1.21) was immobilized in fibroin film by using a photo-crosslinking agent, 4,4'-diazidostilbene-2,2'-disodium sulfonate. Crosslinking and immobilization reactions proceeded by light irradiation for 20 min in air. The immobilized enzyme showed approximately 50% of its native activity with an apparent Michaelis constant of 3.1 mM. The Michaelis constant of the native enzyme was 2.3 mM. Some properties of the immobilized and native enzymes were compared.

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