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Application of partial autoproteolysis to extraction of protein from Antarctic krill (Euphausia superba). Part 3. Changes in and yield of nitrogen substances during autoproteolysis of fresh and frozen krill

✍ Scribed by Kolakowski, E. ;Lachowicz, K.


Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
361 KB
Volume
26
Category
Article
ISSN
0027-769X

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✦ Synopsis


Abstract

Respective utilities of the fresh and frozen (after a 10‐month storage at −21 °C) krill for precipitate production by means of partial autoproteolysis within 10–60 °C were compared. The highest yield of the precipitate was found at about 20 °C, regardless of the raw material used, although the optimal temperature ranges for the fresh and frozen krill were 20–30 °C and 10–20 °C, respectively. The main factors assisting the protein extraction during partial autoproteolysis were enzymatic protein hydrolysis in the fresh krill and the loosening of tissues brought about by water crystallization in the frozen krill.


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Application of partial autoproteolysis t
✍ Kolakowski, E. ;Gajowiecki, L. ;Szybowicz, Z. ;Chodorska, T. 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 409 KB

## Abstract The method of protein extraction from krill using partial autoproteolysis was investigated in this work. Three distinct pH maximal ranges (4.50 to 6.25; 7.75 to 8.50; 9.25 to 9.75) were found to occur at nitrogen substances extractions, depending on pH applied. The highest yield of prot

Application of partial autoproteolysis t
✍ Kolakowski, E. ;Gajowiecki, L. ;Szybowicz, Z. ;Chodorska, T. 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 324 KB

## Abstract Two temperature maxima were found of protein extration using partial autoproteolysis of krill. The extracts obtained at 45‐55 °C show a high content of non‐protein nitrogen, polypeptides and free amino acids, with a low content of thermally precipitated protein. This extract can be util