Application of paper chromatography and electrophoresis to the assay of pharmaceutical products

✍ Scribed by Herbert Schriftman; Robert C. Shultz

• Publisher: John Wiley and Sons
• Year: 1961
• Tongue: English
• Weight: 481 KB
• Volume: 50
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600500412

✦ Synopsis

Results comparable to conventional colorimetric and spectrophotometric methods have been obtained by paper chromatographic and electrophoretic methods. In several instances, where stability studies are involved or when the combination of drugs do not lend themselves to assay by the usual methods, it is concluded that the Chromatographic and/or electrophoretic procedures are a practical applicable solution. Some of the modifications that are needed for applying these chromatographic and electrophoretic methods to pharmaceutical assays are discussed. In general, separations of the various compounds are accomplished by ascending or descending gaper partition chromatography, by selection of appropriate solvent systems, or y paper electrophoresis with selected pH buffer systems. The de- veloped papergrams are sprayed with an appropriate color reagent and assayed quantitatively by photoelectric densitomeuy. The compounds treated in this manner are: phenylephrine hydrochloride, chlorpheniramine maleate, dextromethorphan hydrobromide, N-acetyl-paminophenol, meperidine hydrochloride, atropine, ethinyl estradiol, procaine and tetracaine hydrochlorides.