✦ LIBER ✦

Application of on-line HPLC-FAB mass spectrometry to the analysis of enzymatic digests of ribonuclease B

✍ Scribed by K. Mock; J. Firth; J. S. Cottrell

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 488 KB
Volume: 24
Category: Article
ISSN: 1076-5174
DOI: 10.1002/oms.1210240813

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✦ Synopsis

A tryptic digest of bovine ribonuclease B was analysed by on-line HPLC-FAB mass spectrometry to verify the primary sequence. Peptides obtained by digestion with trypsin before and after treatment with N-glycanase were separated on a slurry-packed fused silica LC column. The entire eluent was introduced into the mass spectrometer source via a continuous flow interface. A single injection of 100 pmoles of material provided data covering over 70% of the sequence.

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