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Application of new, high-sensitivity, 1H13C-N.M.R.-spectral techniques to the study of oligosaccharides

✍ Scribed by Laura Lerner; Ad Bax

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
727 KB
Volume
166
Category
Article
ISSN
0008-6215

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✦ Synopsis

Four n.m.r. methods that are especially useful for characterization of oligosaccharides are applied to the trisaccharide a-NeuSAc-(2+3)-P-Gal-(l-4)-Glc

(1). Three of these are two-dimensional, heteronuclear methods that provide chemical-shift correlation maps having much higher sensitivity than was previously possible, because they rely on indirect observation of 13C via IH detection. These methods are used to assign, completely, the 'H-and 13C-n.m.r. spectra of both anomers of the trisaccharide. In addition to these two-dimensional methods, a onedimensional method is used to measure IH-1H coupling-constants accurately within each sugar ring. The values of the coupling constants thus measured for 1 are evidence that the conformations of the individual sugar rings are not affected by linkage into the trisaccharide.

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