Application of negative ion MS/MS to the identification of N-glycans released from carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1)
✍ Scribed by Dr. David J. Harvey; Kavitha Baruah; Christopher N. Scanlan
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 237 KB
- Volume
- 44
- Category
- Article
- ISSN
- 1076-5174
- DOI
- 10.1002/jms.1470
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✦ Synopsis
Abstract
Structures of N‐glycans released from rat CEACAM1 expressed in human embryonic kidney cells were determined by MALDI and negative ion nanospray MS/MS techniques. The major carbohydrates were bi‐, tri‐ and tetra‐antennary complex glycans with and without sialic acid, fucose and bisecting GlcNAc residues. High‐mannose glycans, predominantly Man~5~GlcNAc~2~, were also found. The negative ion fragmentation technique easily identified the branching pattern of the triantennary glycans (mainly branched on the 6‐antenna) and the presence of ‘bisecting’ GlcNAc residues (attached to the 4‐position of the core mannose), features that are difficult to determine by traditional techniques. Sialic acids were in both α2–3 and α2–6 linkage as determined by MALDI‐TOF MS following linkage‐specific derivatization. Copyright © 2008 John Wiley & Sons, Ltd.